Dura Mater Microvasculature Segmentation in Epifluorescence Microscopy Images


Abstract:

We are currently developing a computerized dura mater laminae analysis system for fluorescence microscopy images of brain tissues from porcine and mice. For this purpose we propose efficient curvilinear segmentation methods (supervised-interactive/unsupervised) for microvasculature detection and extraction in high resolution epifluorescence imagery.

The overall project started in January 2012 and is still ongoing. We proposed robust filtering based segmentation in [1] and proposed a novel fusion based method [2] for further improving the segmentation accuracy. Recently, a random forest classifier driven segmentation is proposed in [3] and the feasibility of a user assisted segmentation is shown in [4].

Related projects: denoising, analysis

Automatic Segmentation Results [1]

(a) Input images, (b) Segmentations


Publications:

[1] V. B. S. Prasath, O. Haddad, F. Bunyak, O. Glinskii, V. Glinsky, V. Huxley, K. Palaniappan. Robust filtering based segmentation and analysis of Dura Mater vessel laminae using epifluorescence microscopy. 35th Annual International Conference EMBS (IEEE EMBS/EMBC), Osaka, Japan. Proc. IEEE, pp. 6055-6058, 2013.

[2] R. Pelapur, V. B. S. Prasath, F. Bunyak, O. V. Glinskii, V. V. Glinsky, V. H. Huxley, K. Palaniappan. Multi-focus image fusion using epifluorescence microscopy for robust vascular segmentation. 36th Annual International Conference EMBS (IEEE EMBS/EMBC), Chicago, USA. Proc. IEEE, pp. 4735-4738, 2014.

Download Poster at figshare: 10.6084/m9.figshare.1147490

[3] Y. M. Kassim, V. B. S. Prasath, R. Pelapur, O. V. Glinskii, R. J. Maude, V. V. Glinsky, V. H. Huxley, K. Palaniappan. Random forests for dura mater microvasculature segmentation using epifluorescence images. 38th Annual International Conference EMBS (IEEE EMBS/EMBC), Orlando, USA, Aug 16-20, 2016. Proc. IEEE, pp. 2901-2904. EMBS Student Paper Competition Finalist!

[4] S. Meena, V. B. S. Prasath, Y. M. Kassim, R. J. Maude, O. V. Glinskii, V. V. Glinsky, V. H. Huxley, K. Palaniappan. Multiquadric spline-based interactive segmentation of vascular networks. 38th Annual International Conference EMBS (IEEE EMBS/EMBC), Orlando, USA, Aug 16-20, 2016. Proc. IEEE, pp. 5913-5916.

[5] Y. M. Kassim, V. B. S. Prasath, O. V. Glinskii, V. V. Glinsky, V. H. Huxley, K. Palaniappan. Microvasculature segmentation of arterioles using deep CNN. IEEE International Conference on Image Processing (ICIP), Beijing, China, Sep 2017. Proc. IEEE.


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